- Title
- Organotypic culture of neonatal murine inner ear explants
- Creator
- Ogier, Jacqueline M.; Burt, Rachel A.; Drury, Hannah R.; Lim, Rebecca; Nayagam, Bryony A.
- Relation
- NHMRC.GNT1023372 http://purl.org/au-research/grants/nhmrc/1023372
- Relation
- Frontiers in Cellular Neuroscience Vol. 13, no. 170
- Publisher Link
- http://dx.doi.org/10.3389/fncel.2019.00170
- Publisher
- Frontiers Research Foundation
- Resource Type
- journal article
- Date
- 2019
- Description
- The inner ear is a complex organ containing highly specialised cell types and structures that are critical for sensing sound and movement. In vivo, the inner ear is difficult to study due to the osseous nature of the otic capsule and its encapsulation within an intricate bony labyrinth. As such, mammalian inner ear explants are an invaluable tool for the study and manipulation of the complex intercellular connections, structures, and cell types within this specialised organ. The greatest strength of this technique is that the complete organ of Corti, or peripheral vestibular organs including hair cells, supporting cells and accompanying neurons, is maintained in its in situ form. The greatest weakness of in vitro hair cell preparations is the short time frame in which the explanted tissue remains viable. Yet, cochlear explants have proven to be an excellent experimental model for understanding the fundamental aspects of auditory biology, substantiated by their use for over 40 years. In this protocol, we present a modernised inner ear explant technique that employs organotypic cell culture inserts and serum free media. This approach decreases the likelihood of explant damage by eliminating the need for adhesive substances. Serum free media also restricts excessive cellular outgrowth and inter-experimental variability, both of which are side effects of exogenous serum addition to cell cultures. The protocol described can be applied to culture both cochlear and vestibular explants from various mammals. Example outcomes are demonstrated by immunohistochemistry, hair cell quantification, and electrophysiological recordings to validate the versatility and viability of the protocol.
- Subject
- organ of Corti; peripheral vestibular organs; dissection; cochlea; hair cell culture; mouse; immunohistochemistry; inner ear
- Identifier
- http://hdl.handle.net/1959.13/1454600
- Identifier
- uon:44975
- Identifier
- ISSN:1662-5102
- Rights
- © 2019 Ogier, Burt, Drury, Lim and Nayagam. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Language
- eng
- Full Text
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